Heat-Labile Enterotoxin, B subunit (LTB) from E. coli
SIGMA/E8656 - recombinant, expressed in Pichia pastoris, >90% (SDS-PAGE), lyophilized powder
Synonym: LTB
Product Type: Chemical
ELISA ELISA plate coated with Monosialoganglioside GM1 from Bovine brain (Cat. No. G7641) incubated with serially diluted Heat-Labile Enterotoxin, B subunit (LTB) from E. coli (Cat. No. E8656) and Anti-Cholera Toxin antibody produced in Rabbit (Cat. No. C3062) followed by Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545) to Determine LTB binding to Monosialoganglioside GM1 by indirect ELISA
| assay | >90% (SDS-PAGE) |
| form | lyophilized powder |
| Quality Level | 200  |
| recombinant | expressed in Pichia pastoris |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Analysis Note: | The activity is measured by ELISA using ganglioside GM1-coated plates (Cat. No. G7641). LTB at various concentrations is incubated on the ganglioside GM1 coated wells, followed by anti-cholera toxin antibody (Cat. No. C3062), and peroxidase-labeled goat anti-rabbit IgG (Cat.No. A0545) as the secondary antibody. Binding saturation of 50% may be achieved with = 0.12 μg/mL of LTB. |
| Application: | Heat-Labile Enterotoxin, B subunit (LTB) from E. coli has been used in enzyme-linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | Enterotoxigenic Escherichia coli causes diarrhea through its heat-labile enterotoxin (LT). The LT is a periplasmic protein composed of one A subunit (LTA, 27 kDa) and five non-covalently associated B subunits (LTB, 11.6 kDa each) forming a ring-like pentamer. LTB has high affinity towards the toxin receptor ganglioside GM1, a glycosphingolipid found ubiquitously on the surface of mammalian cells. Ganglioside GM1 facilitates the delivery of the A subunit to the cytosol of the target cell resulting in persistent synthesis of cAMP and subsequently diarrhea. This characteristic makes LTB a good label for microglial cells (due to the enrichment of ganglioside GM1 on their cell surface). In addition, studies made mostly with animal models demonstrated that recombinant LTB could stimulate strong serum and mucosal immune responses against LT. Other studies have indicated that LTB could be used as a potent mucosal adjuvant. |
| Biochem/physiol Actions: | Heat-Labile Enterotoxin, B subunit (LTB) is a good label for microglial cells. In addition, recombinant LTB stimulates strong serum and mucosal immune responses against heat-labile enterotoxin (LT). Other studies have indicated that LTB could be used as a potent mucosal adjuvant. |
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| Physical form: | Lyophilized from a solution containing Heat-Labile Enterotoxin, B subunit in 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3, and 1 mM sodium EDTA. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H312 - H412 |
| Precautionary statements | P273 - P280 - P302 + P352 + P312 - P362 + P364 - P501 |
| Hazard Codes | T |
| Risk Statements | 24/25-52/53 |
| Safety Statements | 36/37-45-61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >90% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |