Monoclonal Anti-Ezrin antibody produced in mouse
SIGMA/E8897 - clone 3C12, ascites fluid
Synonym: Anti-CVIL; Anti-CVL; Anti-HEL-S-105; Anti-VIL2
MDL Number: MFCD00211933
Product Type: Chemical
Western Blotting JOURNAL CITATION: A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness. By: Grau, L., Luque-Garcia, J. L., et al. in PLoS One, 2013. PubMed ID: 23308193 Image collected and cropped by CiteAb from the following publication, (https://dx.plos.org/10.1371/journal.pone.0053328), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence A431 cells were fixed and permeabilized with 4% Paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:400 Monoclonal Anti-Ezrin, Clone: 3C12 (Cat. No. E8897). The antibody was developed using Goat Anti-Mouse IgG, ATTO 488 conjugate (Cat. No. 62197). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Chicken fibroblast cell extract was separated on SDS-PAGE and probed with Monoclonal AB to Ezrin (MO) CL: 3C12 (Cat. No. E8897). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate. Antibody dilution 1/8,000.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C12, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 80 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, hamster, monkey, kangaroo rat, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1:4,000 using cultured human fibroblast cell line extract | |
| UniProt accession no. | P15311  |
| Application: | The antibody was used for detection of Ezrin by immunostaining, immunoblotting in human bladder cancer cells , immunohistochemistry, immunoprecipitation in kidney and colon adenocarcinoma cells and electron microscopy in astrocytes |
| Biochem/physiol Actions: | Ezrin links the cytoskeleton to the plasma membrane, based on its amino acid sequence homologies with other cytoskeletal proteins. It regulates cell growth, migration, and cell adhesion. Ezrin is also implicated in cancer cell invasion and metastasis. The proteins of the ezrin family serve as tyrosine kinase phosphorylation substrates. They exhibit a growth factor receptor-specific pattern of phosphorylation. Ezrin is phosphorylated by epidermal growth factor receptor and platelet-derived growth factor receptor. |
| Biochem/physiol Actions: | Monoclonal Anti-Ezrin recognizes ezrin (80 kDa). Cross-reactivity is observed with human, monkey, bovine, rat, kangaroo, hamster, and mouse |
| Biochem/physiol Actions: | The ezrin/radixin/moesin (ERM) family regulates the dynamic cell membrane functions such as endocytosis, exocytosis, and transmembrane and other cortical signalling pathways. Ezrin (molecular weight 80 kDa) interacts with transmembrane proteins and F-actin and mediate important signalling pathways including RhoA and Hedgehog signalling. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ezrin is a member of the best studied member of the ERM family of proteins. The ERM family regulates the dynamic cell membrane functions such as endocytosis, exocytosis, and transmembrane and other cortical signalling pathways. Ezrin (molecular weight 80 kDa) interacts with transmembrane proteins and F-actin and mediate important signalling pathways including RhoA and Hedgehog signalling. Ezrin is also present in the microvillus surface of acid-secreting parietal cells of the gastric glands, where it presents as a family of isoelectric variants. By immunoelectron microscopy of human choriocarcinoma cells, ezrin is enriched just inside the plasma membrane of microvilli and less abundant on more planar aspects of the membrane. |
| General description: | Monoclonal Anti-Ezrin (mouse IgG1 isotype) is derived from the 3C12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Ezrin is present in microvilli, placental microvilli, microspikes, and membrane ruffles of cultured cells. It is also found in the growth cones of cultured neurons and the marginal band of avian erythrocytes. Ezrin comprises a (4.1 protein, ezrin, radixin and moesin) FERM domain, an α-helical linker region and a C-terminal domain. The amino-terminal domain of ezrin is localized to membranes and the carboxy-terminal part, containing the α-helical domain, colocalizes with actin filaments. Ezrin gene is mapped to the human chromosome location 6q25.3. |
| Immunogen: | carboxy-terminal section of recombinant human ezrin (amino acids 362-585). |
| Physical form: | Monoclonal Anti-Ezrin is provided as ascites fluid with 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |