Phosphodiesterase 5A1 human
SIGMA/E9034 - recombinant, expressed in Sf9 cells
Synonym: 3′,5′-cyclic-GMP phosphodiesterase; PDE 5A1
Product Type: Chemical
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| form | solution |
| mol wt | 126 kDa |
| Quality Level | 200  |
| recombinant | expressed in Sf9 cells |
| shipped in | dry ice |
| specific activity | ≥2,000 unit/μg protein |
| storage temp. | −70°C |
| UniProt accession no. | O76074  |
| Biochem/physiol Actions: | PDE5 regulates vascular smooth muscle contraction and is involved in NO-cGMP signaling in platelets to control aggregation. PDE5 may also be involved in the regulation of cGMP signaling in the brain and may modulate pressure-induced cardiac hypertrophy and fibrosis . Phosphodiesterase (PDE)is an enzyme that is used to break phosphodiester bonds. Phosphodiesterase 5 (PDE5), amolecular target for the treatment of erectile dysfunction and pulmonaryhypertension. PDE5 is used to find novel PDE5 inhibitors. PDE5 specifically targets cyclicguanosine monophosphate (cGMP) generated by the activation of nitric oxide (NO). PDE5 regulates cGMP signalingin the cardiovascular and other tissues. At the genetic level PDE5 isexpressed as various isoforms and undergoes phosphorylation as a post-translationalmodific |
| General description: | N-terminal GST-tagged 126 kDa full-length protein |
| General description: | Research area: Cell SignallingPDE5is located in various tissues such as the kidney, brain, lungs, cardiac myocytes,vascular smooth muscle cells, penile corpus cavernosum, platelets, andgastrointestinal tissues. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Other Notes: | One unit will convert 1.0 picomole of 3′,5′-cGMP to 5′-GMP per minute at pH 7.4 at 37 °C. |
| Physical form: | Supplied as a solution in 20% glycerol containing 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% TWEEN® 20 and 3 mM DTT |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2,000 unit/μg protein |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 3.1.4.25 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |