EphB2/Fc Chimera from mouse
SIGMA/E9402 - >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
Synonym: Cek5; Drt; ERK; Hek5; Nuk; Qek2; Sek3; Tyro5
MDL Number: MFCD01867526
Product Type: Chemical
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| assay | >90% (SDS-PAGE) |
| biological source | mouse |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 100-110 kDa by SDS-PAGE (reducing) |
| monomer calculated mol wt 85.3 kDa | |
| packaging | pkg of 200 μg |
| potency | 0.04-2.5 ng/mL |
| Quality Level | 100  |
| recombinant | expressed in NSO cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | ligand binding assay: suitable |
| UniProt accession no. | P54763  |
| Analysis Note: | The biological activity is measured by its ability to bind recombinant mouse ephrin-B2/Fc in an ELISA assay. |
| Application: | EphB2/Fc Chimera from mouse has been used for the investigation of the role of ephrinB-EphB signaling in the induction and maintenance of hyperalgesia, hyperexcitability of neurons of DRG (dorsal root ganglion) and hyperexcitability and elevated synaptic plasticity of neurons of DH (dorsal horn) regions of brain. |
| Biochem/physiol Actions: | EphB2 (Epherin receptor B2) is involved in the development and plasticity of glutamatergic synapses, and their interaction with NMDA receptor. It can also regulate the processing of acute inflammatory pain, and mechanical allodynia as a result of nerve crush. Activation of this receptor results in recruitment and activation of Src-protein kinases. These, in turn, phosphorylate and activate NMDA receptor. It regulates the function of platelets and the development of thrombus. It is also responsible for the retraction of clots, and modulates independent platelet function in a contact-independent manner. EphB2 signaling is also involved in the modulation of axonal sprouting, which is induced by lesions. |
| Biochem/physiol Actions: | Member of the Eph receptor tyrosine kinase family shown to bind ephrin-B1, ephrin-B2, and ephrin-B3; involved in pattern formation and morphogenesis, and in arterial/venous demarcation of the developing vasculature. |
| General description: | EphB2 (Epherin receptor B2) is a receptor tyrosine kinase (RTK), which belongs to a 13 member family of ephrin receptors. This family is divided into two classes A and B. EphB2 belongs to EphB group, which contains five members. The ligands to this group of receptors are named ephrin-B1-B3. It is a bidirectional functioning membrane protein. |
| Other Notes: | Extracellular domain of mouse EphB2 (amino acids 1-548) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.0. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |