Erythropoietin from mouse
SIGMA/E9530 - recombinant, expressed in NSO cells, ≥90% (SDS-PAGE), lyophilized powder, suitable for cell culture
Synonym: EPO
MDL Number: MFCD01867534
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| biological source | mouse |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | ~36 kDa by SDS-PAGE |
| predicted mol wt 18.6 kDa | |
| packaging | pkg of 10 μg |
| potency | 1.030 ng/mL |
| Quality Level | 200  |
| recombinant | expressed in NSO cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P07321  |
| Analysis Note: | The biological acitivity is measured by its ability to stimulate cell proliferation using TF-1 cell line. |
| Biochem/physiol Actions: | EPO (erythropoietin) plays an essential role in the growth and differentiation of erythrocyte progenitor cells. It is involved in the pathogenesis of myomatous erythrocytosis syndrome, which is characterized by erythrocytosis and myomatous uterus. It acts as a ligand for a homodimer of EPO-receptor (Epo-R), which is localized to the surface of immature erythroid cells. This interaction leads to inhibition of apoptosis and facilitation of cell proliferation and differentiation to mature erythrocytes. |
| Biochem/physiol Actions: | Erythropoietin is a glycoprotein that is the principal regulator of red blood cell growth and differentiation. |
| General description: | EPO (erythropoietin) gene is localized to human chromosome 7q22. This protein is predominantly produced by the cortex cells of kidney, as well as a variety of tissues including female reproductive organs. It is a glycoprotein, which has a molecular weight of 34kDa. It is produced as a 193-amino acid precursor protein, which is cleaved to produce a secreted protein of 166-amino acid. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |