Anti-Endothelial Cells antibody, Mouse monoclonal
SIGMA/E9653 - clone P1H12, purified from hybridoma cell culture
Synonym: Anti-CD146
MDL Number: MFCD03792419
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Pig tongue were stained with 1:100 Anti-Endothelial Cells antibody, Mouse monoclonal, Clone: P1H12 (Cat. No. E9653), followed by 40 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B0529) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | P1H12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 130 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable using 2% paraformaldehyde 9:1 methanol |
| immunocytochemistry: suitable using 4% paraformaldehyde 0.1-0.5% triton X-100, smears or live cells | |
| immunohistochemistry: suitable using using acetone-fixed sections of human tongue | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable |
| Application: | Anti-Endothelial Cells antibody, Mouse monoclonal has been used in: • immunocytochemistry • immunohistochemistry • flow cytometry • immunoprecipitation • enzyme-linked immunosorbent assay (ELISA) • western blotting |
| Biochem/physiol Actions: | CD146 (cluster of differentiation 146) expression can stimulate tumor development in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells during implantation and placentation, thus limiting trophoblastic invasion in the myometrium. |
| Biochem/physiol Actions: | Recognizes cultured microvascular and large-vessel endothelial cells (MVEC and HUVEC, respectively) and endothelial cells in tissues and in circulation. |
| Biochem/physiol Actions: | Soluble CD146 (cluster of differentiation 146) is associated with the activation of angiogenesis. Circulating endothelial cells (CECs) may help in predicting clinical outcome in cancer patients. Tumor endothelial markers are expressed in a wide range of tumor types and in normal vessels associated with wound healing and corpus luteum formation. It may have significant implications for the development of anti-angiogenic therapies as well. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Endothelial cells (CD146) (mouse IgG1 isotype) is derived from the P1H12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with human umbilical cord cells (HUVEC). CD146 (cluster of differentiation 146) is also known as the melanoma cell adhesion molecule (MCAM), cell surface glycoprotein MUC18, A32, Mel-CAM and S-Endo-1. It is encoded by the MCAM gene. It belongs to the immunoglobulin supergene family with five immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. It is located on human chromosome11q23. |
| Immunogen: | human umbilical cord cells (HUVEC). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Safety Statements | 23-24/25 |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |