Endoglycosidase F1 from Elizabethkingia miricola
SIGMA/E9762 - recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution
Synonym: Endo F1; Endo-β-N-acetylglucosaminidase F1; Endoglycosidase F1 from Chryseobacterium meningosepticum; Endoglycosidase F1 from Elizabethkingia meningoseptica; Endoglycosidase F1 from Flavobacterium meningosepticum
MDL Number: MFCD00146843
Product Type: Chemical
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| conjugate | (N-linked) |
| form | buffered aqueous solution |
| mol wt | 32 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| specific activity | ≥16 U/mg |
| storage temp. | 2-8°C |
| Application: | Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides. |
| Application: | Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR) and to glycosylate fucosylated N-glycopeptides. |
| Biochem/physiol Actions: | Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage. |
| General description: | Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme. |
| Other Notes: | One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5. |
| Packaging: | Supplied with 5× Reaction Buffer, 250 mM NaH2PO4, pH 5.5. |
| Physical form: | Aseptically filled solution in 20 mM Tris-HCl pH 7.5 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥16 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.96 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |