SOLu-Trypsin
SIGMA/EMS0004 - recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid
Synonym: Protein Digestion for Mass Spectrometry; Ready to Use Recombinant Trypsin for Protein Digestion in Mass Spectrometry; Recombinant Trypsin for Protein Digestion in Mass Spectrometry; Trypsin for Protein Digestion; Trypsin for Protein Digestion; rTrypsin
Product Type: Chemical
100 μg vial. Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| description | Activity:≥ 10,000 unit/mg protein |
| form | ready-to-use solution |
| grade | Proteomics Grade |
| Quality Level | 200  |
| recombinant | expressed in Pichia pastoris |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| suitability | suitable for mass spectrometry |
| UniProt accession no. | P00761  |
| Application: | Poster: Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays |
| Application: | SOLu-Trypsin: • has been used to digest the gel pieces for mass spectrometry identification of active deubiquitnases (DUBs) • has been used in trypsin sensitivity assay • may be used for the digestion of wine proteins |
| Biochem/physiol Actions: | • SOLu-Trypsin (EMS0004) is our exclusive, Advanced Proteomics Grade enzyme that is solution-stable for mass spectrometry. • Designed to be stable in solution when refrigerated, SOLu-Trypsin can be used immediately without preparation. • Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately. • SOLu-Trypsin allows excess product to be saved for future use, thus eliminating unnecessary waste and cost. • It is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion. |
| Biochem/physiol Actions: | Trypsin is used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. It hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. |
| Features and Benefits: | • Ready to use - no preparation, such as reconstitution or thawing, is required • Fits seamlessly into established workflow - no need to modify protocols • Eliminates waste - remains stable in the refrigerator after use so there is no need to discard excess product • Recombinant, porcine sequence - no chymotryptic activity • Stable for short-term use at room temperature in an autosampler or on a liquid handling robot |
| General description: | Trypsin is a pancreatic serine endoprotease. SOLu-Trypsin is made from recombinant trypsin, porcine sequence. It does not possess chymotryptic activity. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |