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Fast Blue RR Salt

SIGMA/F0500 - crystalline

Synonym: 4-Benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt; Azoic Diazo No. 24

CAS Number: 14726-29-5
Empirical Formula (Hill Notation): C15H14ClN3O3 · 0.5ZnCl2
Molecular Weight: 387.89
EC Number: 238-777-5
MDL Number: MFCD00074762
Linear Formula: C15H14ClN3O3 · 1/2 ZnCl2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-F0500-25G 25 g
 $598.00
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F0500-25G

 

εmax ≥15000 at 326-332  nm
application(s) diagnostic assay manufacturing
hematology
histology
form crystalline
InChI 1S/2C15H13N3O3.4ClH.Zn/c2*1-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2
InChI key LLARVKAOQLUCQI-UHFFFAOYSA-L
Quality Level 200 
SMILES string [Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4
solubility water: 1 mg/mL, clear to hazy, yellow-green
storage temp. −20°C
Application: Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.
Biochem/physiol Actions: Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appears black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.
Packaging: 25 g in glass bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
Colour Index Number 37155
UNSPSC 12171500

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