Fetal Bovine Serum
SIGMA/F0926 - USDA approved, sterile-filtered, suitable for cell culture
Synonym: Fetal Bovine Sera; Fetal Calf Sera; FBS; FCS; sera; serum
MDL Number: MFCD00132239
Product Type: Chemical
| biological source | bovine fetus |
| composition | Hemoglobin, ≤25 mg/dL |
| impurities | ≤10 EU/mL endotoxin |
| product line | FBS Classic |
| quality | USDA approved |
| Quality Level | 500  |
| shipped in | dry ice |
| sterility | sterile-filtered |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | • Endotoxin and hemoglobin tested |
| Analysis Note: | • Tested for the presence of bacteria, virus, and mycoplasma • Triple filtered with 0.1 micron membrane under aseptic conditions |
| Application: | FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factors to cells in culture. Fetal Bovine Serum has been used for the following cell cultures: • Primary Culture Human Macrophages (mononuclear cells were re-suspended in Roswell Park Memorial Institute (RPMI) medium containing fetal bovine serum (FBS) along with other components). • Tissue Culture (MEFs were cultured in DMEM supplemented with FBS along with components). • Isolation and 3D culture of primary mammary epithelial organoids • Cell culture and medium (HUVECs were grown in Endothelial Cell Growth Medium-2 supplemented with FBS and growth factors) |
| General description: | Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood. It comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. It is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Suitability: | USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |