Follistatin 300 human
SIGMA/F1175 - ≥90% (SDS-PAGE), recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture
Synonym: FS 300
MDL Number: MFCD02098540
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 31 kDa |
| packaging | pkg of 25 μg |
| potency | 0.1-0.4 mg per mL |
| Quality Level | 100  |
| recombinant | expressed in Sf21 cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P19883  |
| Analysis Note: | The biological activity is measured by its ability to neutralize activin-induced bioactivity on K562 cells (erythroid differentiation). |
| Application: | FST (follistatin) has been used for trophoblast fusion assay and the measure of hCG (human chorionic gonadotropin) concentration in hormone assays. It is also suitable for the development of chondrocytes from hESc (human embryonic stem cells) by a new 3-Stage directed differentiation protocol (DDP). |
| Biochem/physiol Actions: | FST (follistatin) binds to and regulates activins, which in turn are TGF (transforming growth factor)-β superfamily members. The expression level of FST and its binding partner activin A is elevated in inflammatory disorders. The activin A-follistatin system plays an essential role in the modulation of glucose and lipid metabolism, which might have an overall effect on fetal growth. In adipose tissue, it facilitates the adipogenic differentiation of progenitor cells. |
| Biochem/physiol Actions: | High-affinity activin-binding protein that can act as an activin antagonist. |
| General description: | FST (follistatin) is an activin-binding protein, and exists in two isoforms due to alternate splicing, FS288 and FS315. It is a glycoprotein with a single chain, and acts as an activin antagonist. It has a high level of expression in fetal membranes and placenta. |
| Packaging: | 25 μg in glass insert |
| Physical form: | Lyophilized from a solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 1.25 mg bovine serum albumin. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H315 - H318 |
| Precautionary statements | P280 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |