Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat
SIGMA/F2012 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (whole molecule)
MDL Number: MFCD00162642
Product Type: Chemical
Immunofluorescence : SCK-CEN Mouse cortex primary neurons cultured for 10 days were stained for PSD95, Β-Tubulin III and nuclei. For PSD95 Monoclonal Anti-PSD95 antibody produced in Mouse (Cat. No. P246) and Anti-Mouse IgG (whole molecule)–FITC antibody produced in Goat (Cat. No. F2012) (green) were used. For Β-Tubulin III Anti-Β-Tubulin III antibody produced in Rabbit (Cat. No. T2200) and Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in Goat (Cat. No. T6778) (red). Nuclei were stained with Hoechst B2883 (blue/purple). Submitted by Dr G. Pani, Studiecentrum voor Kernenergie-Centre d′Etude d′Energie Nucleaire
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:64 (minimum) |
| Application: | Anti-Mouse IgG (whole molecule)−FITC antibody has been used: • in immunofluorescence • in immunohistochemistry • in immunofluorescence staining • in immunofluorescence microscopy • in confocal imaging |
| Application: | Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat was used for FACS analysis of DF3 antigen on breast cancer cell lines. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti-Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| General description: | The product binds all mouse Igs. |
| Other Notes: | Antibody adsorbed with human serum proteins. |
| Packaging: | 0.5 mL in glass insert |
| Packaging: | 1, 2 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with human samples |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |