Anti-β-Tubulin−FITC antibody, Mouse monoclonal
SIGMA/F2043 - clone TUB 2.1, purified from hybridoma cell culture
Synonym: Monoclonal Anti-β-Tubulin
Product Type: Chemical
Immunofluorescence Chicken fibroblasts were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 1:50 Monoclonal Anti-β-Tubulin–FITC Clone: TUB 2.1 (Cat. No. F2043).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TUB 2.1, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | direct immunofluorescence: 1:25 using cultured BHK cells |
| Application: | FITC-β tubulin antibody may be used for detection ajnd localization of β-tubulin by direct labeling in a variety of eukaryotic cells ranging from human embryonic kidney cells (HEK293T) to cells of azuki bean epicotyl. by immunohistochemical and immunocytochemical assays. |
| Application: | Monoclonal Anti-β-Tubulin-FITC antibody has been used: • in the evaluation of the status of the meiotic spindle and the arrangement of chromosomes • in immunofluorescence studies • in immunofluorescence microscopy • in spindle and chromosome imagery(6) |
| Biochem/physiol Actions: | Mutation in β-Tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure. |
| General description: | β-Tubulin belongs to the subfamily of tubulins. It has two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain, which has the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)). TUBB1 (tubulin beta class I) is located on human chromosome 6p21.33.(10) |
| Immunogen: | tubulin from rat brain. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide. |
| Specificity: | The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |