EZview™ Red ANTI-FLAG® M2 Affinity Gel
SIGMA/F2426 - clone M2
Synonym: Monoclonal ANTI-FLAG® M2; Anti-ddddk; Anti-dykddddk
Product Type: Chemical
| analyte chemical class(es) | proteins |
| capacity | ≥0.6 mg/mL, gel binding capacity |
| clone | M2, monoclonal |
| isotype | IgG1 |
| matrix | 4% agarose bead; 45-165μm bead size |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | affinity chromatography: suitable |
| immunoprecipitation (IP): suitable |
| Application: | Immunoprecipitation (IP) of FLAG- and 3xFLAG-tagged fusion proteins. Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptide Learn more product details in our FLAG® application portal. |
| Biochem/physiol Actions: | Suitable for purifying N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins. |
| Disclaimer: | FLAG® Affinity Gels, FLAG® tag, 3xFLAG®tag, DYKDDDDK tag |
| General description: | EZ view Red Anti-FLAG M2 Affinity Gel is a resin that consists of Anti-FLAG M2 antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind FLAG fusion proteins to samples, such as cell lysates and tissue, for purification of FLAG-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. Agarose beads bind at N-terminal, Met-N-terminal and C-terminal FLAG fusion proteins, 3xFLAG-tagged fusion proteins. |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | EZview is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | 1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |