Monoclonal ANTI-FLAG® M2 antibody produced in mouse
SIGMA/F3165 - clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
Synonym: Anti-ddddk; Anti-dykddddk
Product Type: Chemical
| antibody form | purified immunoglobulin (Purified IgG1 subclass) |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M2, monoclonal |
| concentration | 3.8-4.2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide) |
| immunogen sequence | DYKDDDDK |
| isotype | IgG1 |
| purified by | using Protein A |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | all |
| storage temp. | −20°C |
| technique(s) | western blot: 10 μg/mL (Protein A) |
| Application: | Monoclonal ANTI-FLAG® M2 antibody produced in mouse has been used in: • immunoblotting • immunoprecipitation • immunocytochemistry • immunofluorescence • ELISA • EIA • chromatin immunoprecipitation • electron microscopy • flow cytometry • supershift assays Browse additional application references in our FLAG® Literature portal. |
| General description: | Anti-Flag M2 antibody is used for the detection of Flag fusion proteins. This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent. F3165 is affinity purified using Protein A resin, so it contains not only the anti-FLAG M2 antibody but also small amounts of native mouse IgG, increasing its sensitivity in most applications. Method of purification - Protein A |
| Immunogen: | FLAG; peptide sequence DYKDDDDK |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 5 mg in poly bottle |
| Preparation Note: | Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk |
| Storage and Stability: | Store the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |