Fetal Bovine Serum

SIGMA/F4135 - USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Synonym: HI FBS; HI FCS; HI sera; HI serum; FBS; FCS; sera; serum

MDL Number: MFCD00132239
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-F4135-100ML	100 mL	$178.00 1/EAAdd To Favorites		Add To Favorites
45-F4135-500ML	500 mL	$782.00 1/EAAdd To Favorites		Add To Favorites
45-F4135-6X500ML	500 mL	$4460.00 6/EAAdd To Favorites		Add To Favorites
45-F4135-24X500ML	500 mL	$17570.00 24/EAAdd To Favorites		Add To Favorites
45-F4135-1L	1 L	$1530.00 1/EAAdd To Favorites		Add To Favorites

biological source	bovine fetus
composition	Bovine IgG, ≤1 mg/mL
	Hemoglobin, ≤20 mg/dL
impurities	≤10 EU/mL endotoxin
origin	USA origin
product line	FBS Classic
Quality Level	500
shipped in	dry ice
sterility	sterile-filtered
storage temp.	−20°C
technique(s)	cell culture \| hybridoma: suitable
	cell culture \| insect: suitable
	cell culture \| mammalian: suitable

Analysis Note:	• Endotoxin and hemoglobin tested
Analysis Note:	• Tested for the presence of bacteria, virus, and mycoplasma • Triple filtered with 0.1 micron membrane under aseptic conditions
Application:	FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture. Fetal Bovine Serum has been used: • as a component of Dulbecco’s Modified Eagles Media (DME, DMEM), Eagle′s Minimum Essential Medium (EMEM), Roswell Park Memorial Institute medium (RPMI-1640), α-modified eagle medium (αMEM) (MEM) and Leibovitz L-15 medium • as a component of blocking medium for treating brainstem slices • to improve attachment of dermal explants
General description:	Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation. It also protects cells from oxidative damage and apoptosis. FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.
Preparation Note:	Prepared by heating at 56°C for 30 minutes.
Preparation Note:	This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.