Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit
SIGMA/F4137 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Rabbit Anti-Chicken IgY (IgG) (whole molecule)
MDL Number: MFCD00162224
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded, Human tonsil tissue sections were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Chicken, followed by 1:200 Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F4137).
Immunofluorescence Human Periferal Blood Lymphocytes probed and developed using 1:100 Anti-Human IgG (whole molecule) antibody produced in Chicken followed by 1:200 Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F4137).
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 |
| indirect immunofluorescence: 1:200 |
| Application: | Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used: • in indirect immunofluorescence • in western blotting • in immunocytochemistry |
| Biochem/physiol Actions: | Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration. |
| Biochem/physiol Actions: | IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa. |
| General description: | Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications . |
| Immunogen: | Chicken IgY (IgG) |
| Immunogen: | Purified chicken IgY (IgG) |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |