Anti-Human Polyvalent Immunoglobulins (whole molecule)−FITC antibody produced in rabbit
SIGMA/F4637 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Rabbit Anti-Human Polyvalent Immunoglobulins (whole molecule)
MDL Number: MFCD00239694
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:160 Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F4637).
Immunofluorescence Rat Liver sections for ANA (anti-nuclear antibody assays) test were probed and developed using 1:400 Human anti-Nuclear (ANA)-Homogeneous and 1:400 Human anti-Nuclear (ANA)-Nucleolar followed by 1:128 Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F4637).
Immunofluorescence Human Periferal Blood Lymphocytes probed and developed using 1:128 Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F4637).
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ANA-indirect immunofluorescence: 1:128 |
| direct immunofluorescence: 1:80 |
| Application: | Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a working antibody dilution of 1:128. For anti-nuclear antibody assay of acetone-fixed rat liver cells, a minimum dilution of 1:128 is recommended. For direct labeling of human tonsil sections, a dilution of 1:80 may be used. This antibody was used to stain C2C12 cell line derived from mouse myoblasts at a dilution of 1:100 |
| Application: | Anti-Human Polyvalent Immunoglobulins (whole molecule)−FITC antibody produced in rabbit has been used in immunohistochemical analysis and immunofluorescence |
| Biochem/physiol Actions: | IgG (immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody participates in complement fixation and opsonization. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens Purified human IgA, IgG and IgM are used to develop anti-sera in rabbits. The IgG fraction of each anti-sera is purified and conjugated to Fluorescein Isothiocyanate (FITC), in an alkaline reaction. Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody specifically recognizes human IgA, IgG and IgM. |
| Immunogen: | Purified human IgA, IgG, and IgM |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |