Monoclonal Anti-Fibrinogen antibody produced in mouse
SIGMA/F4639 - clone FG-21, ascites fluid
MDL Number: MFCD00162408
Product Type: Chemical
ELISA Monoclonal Anti-Fibrinogen antibody produced in Mouse, Clone: FG-21 (Cat. No. F4639), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Fibrinogen by indirect ELISA.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FG-21, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, baboon |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:4,000 |
| UniProt accession no. | P02671  |
| Application: | Monoclonal Anti-Fibrinogen antibody produced in mouse has been used in: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • fibrinogen adsorption assay |
| Biochem/physiol Actions: | Fibrinogen, a blood coagulation protein, is regarded as the central protein in the blood coagulation system.Fibrinogen plays a crucial role in protecting the vascular network against the loss of blood after tissue injury. |
| Biochem/physiol Actions: | Plasmin attacks the Aα chain COOH domain to produce the heterogeneous fragment X. After a series of degradation, it ended with terminal digestion products: fragments D and E which represent the major globular domains in fibrinogen. Mutations in this gene leads to several disorders including hypofibrinogenemia, afibrinogenemia. |
| Biochem/physiol Actions: | The antibody is specific for human fibrinogen and recognizes an epitope sensitive to reduction commonly used in immunoblotting assays. It also reacts with synthetic human fibrinopeptides A and B, fibrinogen related peptides and baboon fibrinogen. No cross-reactivity with bovine, pig, sheep, horse, dog, goat, rabbit, guinea pig, or rat is observed. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Fibrinogen, is a thrombin-coagulable soluble plasma 340kDa glycoprotein, composed of paired sets of three subunits i.e. α, β, γ. Among three subunits, β and γ subunits contain one N-glycosylation site, which is occupied by a biantennary N-glycan. It contains three pairs of disulfide bonded chains called α, β, γ which further folded into four structural domains: the D, E, connector and the COOH-terminal region of the Aα chain. |
| General description: | Monoclonal Anti-Fibrinogen (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | purified haptoglobin from pooled human plasma containing the three major haptotypes. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |