Filipin III from Streptomyces filipinensis
SIGMA/F4767 - ≥85% (HPLC)
Synonym: Filimarisin
CAS Number: 480-49-9
Empirical Formula (Hill Notation): C35H58O11
Molecular Weight: 654.83
MDL Number: MFCD00466923
Linear Formula: C35H58O11
Product Type: Chemical
Chinese Hamster Ovary (CHO) cells stained with Filipin III (F4767). Filipin III was dissolved at 25 mg/mL in DMSO and was further diluted to a working solution at a concentration of 0.05 mg/mL in PBS/10%FBS. After fixation with paraformaldehyde cell were incubated for 2 hours with Filipin III working solution and images were acquired using UV filter set (340-380 nm excitation, 40 nm dichroic, 430 nm long pass filter).
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F4767-1MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F4767-5MG
| antibiotic activity spectrum | fungi |
| yeast | |
| assay | ≥85% (HPLC) |
| form | powder |
| InChI | 1S/C35H58O11/c1-4-5-11-16 |
| InChI key | IMQSIXYSKPIGPD-BWJOQPJXSA |
| mode of action | cell membrane | interferes |
| mp | 163-180 °C |
| Quality Level | 300  |
| SMILES string | CCCCCC(O)C1C(O)CC(O)CC(O) |
| solubility | methanol: 9.80-10.20 mg/mL, clear, colorless to yellow |
| storage temp. | −20°C |
| Application: | Filipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. |
| Application: | Filipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex. |
| Biochem/physiol Actions: | Filipin is a polyene macrolide antibiotic and antifungal. The antifungal mechanism of action may be due to altering membrane permeability and associated functions by binding to membrane sterols. Filipin inhibits prion protein (PrP) endocytosis and causes the release of PrP from the plasma membrane. |
| Other Notes: | Filipin III is the major component of 8 isomeric components in filipin. |
| Packaging: | 1, 5 mg in glass bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (HPLC) |
| mp | 163-180 °C |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |