Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse
SIGMA/F4771 - clone 1B10, ascites fluid
Synonym: Fibroblast Surface Protein Antibody; Fibroblast Surface Protein Antibody - Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse
MDL Number: MFCD00164646
Product Type: Chemical
Immunofluorescence Hs68 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 Anti-Fibroblast Surface Protein antibody, Mouse monoclonal, Clone: 1B10 (Cat. No. F4771). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B10, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| mol wt | antigen 43-72-80 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | complement-mediated cytotoxicity assay: 1:500 |
| flow cytometry: suitable | |
| immunohistochemistry (frozen sections): suitable |
| Analysis Note: | Working dilution is at least 1:500 by complement-mediated microcytotoxicity assay. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) Immunohistochemistry (1 paper) |
| Application: | Monoclonal anti-fibroblast surface protein antibody can be used in flow cytometry of human fibroblasts and monocytes. It is also useful in immunoblotting and western blotting. |
| Application: | Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse has been used in: • immunofluorescence • flow cytometry • immunohistochemistry • immunoblotting |
| Biochem/physiol Actions: | Monoclonal Anti-Fibroblast Surface Protein antibody is also used to remove fibroblasts from human cultured cells by inhibiting fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human Fibroblast Surface Protein (mouse IgM isotype) is derived from the 1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cultured human thymic fibroblasts. Human fibroblast surface protein is a fibroblast antigen that is expressed mainly on human synovial, foreskin and thymic fibroblasts and is absent on human epithelial cells and lymphocytes. |
| General description: | This fibroblast antigen has been demonstrated on human synovial, mammary, foreskin and thymic fibroblasts and in malignant fibrosarcoma tissue. The antigen is both cell membrane and lysosome associated. |
| Immunogen: | cultured human thymic fibroblasts |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |