Monoclonal Anti-Cytokeratin Peptide 18−FITC antibody produced in mouse
SIGMA/F4772 - clone CY-90, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-Cytokeratin Peptide 18
MDL Number: MFCD00162250
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Immunofluorescence confocal laser scanning microscopy and immuno-electron microscopic identification of keratins in human materno-foetal interaction zone. By: Ahenkorah, J., Hottor, B., et al. in J Cell Mol Med, 2009. PubMed ID: 18466353 Image collected and cropped by CiteAb from the following publication, (http://doi.wiley.com/10.1111/j.1582-4934.2008.00363.x), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin fixed, paraffin-embedded Human Placenta tissue sections were stained with 1:100 Monoclonal Anti-Cytokeratin Peptide 18-FITC antibody produced in Mouse, Clone: CY-90 (Cat. No. F4772).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CY-90, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | wide range |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded sections of human or animal tissues |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| Application: | Monoclonal Anti-Cytokeratin Peptide 18 FITC antibody produced in mouse has been used in: • direct immunofluorescent staining • immunohistochemistry • double immunofluorescence • immunocytochemical and immuno?electron microscopic localization of keratins |
| Application: | Monoclonal Anti-Cytokeratin Peptide 18-FITC antibody: • is suitable for immunohistochemistry to identify renal tubular epithelial cells in the extrarenal cells regeneration during acute renal failure • is suitable for immunocytochemical and immuno-electron microscopic localisation of keratins in human materno-foetal interaction zone • may be used for single or double labeling procedures |
| Biochem/physiol Actions: | Cytokeratins also called keratins (Ks) according to the new nomenclature system.These are known to be the major structural proteins of epithelial cells. It is heteropolymeric in nature i.e. in cells more than one keratin protein is always present. It belongs to type I acrylic sub-family and is differentially expressed in various human tissues. It maintains the structural integrity of extra-embryonic membrane epithelia. Broadly keratins are grouped into two types on the basis of their biochemical and immunological properties: Type II keratins are a family of basic proteins identified as K1 to K8, whereas type I keratins consists of K9 to K20, belong to the acidic group. |
| Biochem/physiol Actions: | Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. FITC Monoclonal Anti-Cytokeratin Peptide 18 is a chain specific antibody which can facilitate typing of normal, metaplastic and neoplastic cells. Cytokeratin 18 (CYK18) is a cytoskeletal protein associated with apoptosis. CK-18 is considered as a promising plasma biomarker to identify patients with HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome. |
| Disclaimer: | For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Broadly keratins are grouped into two types on the basis of their biochemical and immunological properties: Type II keratins are a family of basic proteins identified as K1 to K8, whereas type I keratins consists of K9 to K20, belong to the acidic group. It belongs to type I acrylic sub-family and is differentially expressed in various human tissues. |
| General description: | FITC Monoclonal Anti-Cytokeratin Peptide 18 is a purified mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) isomer I. Monoclonal Anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the CY-90 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice. Cytokeratin 18 (CYK18) is a 45 kDa polypeptide, differentially expressed in various human tissues. |
| Immunogen: | human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines. |
| Physical form: | Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA with 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |