Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse
SIGMA/F4890 - clone RG-16, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635) followed by 1:25 Monoclonal Anti-Rabbit Immunoglobulins-FITC antibody produced in Mouse, Clone: RG-16 (Cat. No. F4890).
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | RG-16, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 |
| indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit |
| Application: | Monoclonal Anti-Rabbit Immunoglobulins-FITC antibody produced in mouse has been used in: • immunocytochemistry • immunohistochemistry • immunofluorescence |
| Application: | The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature. |
| Biochem/physiol Actions: | Binds only rabbit Igs. Does not react with reduced rabbit Igs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM. |
| Physical form: | Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |