Anti-Ferritin, Human antibody produced in rabbit
SIGMA/F5012 - whole antiserum, liquid
Synonym: Anti-FHC; Anti-FTH; Anti-FTHL6; Anti-HFE5; Anti-PIG15; Anti-PLIF
MDL Number: MFCD00162407
Product Type: Chemical
Immunohistochemical staining of oligodendrocytes in the globus pallidus of developing (8 weeks) rat brain. Rat brain sections are stained with Rabbit Anti-Ferritin (Cat. No. F5012) and ExtrAvidin® Peroxidase Staining Kit (Cat. No. EXTRA-3) with diaminobenzidine (DAB)/nickel enhanced staining. Magnification 1000x. From Dr. S. Shoham, Herzog Hospital, Dept of Research, Jerusalem, Israel.
Immunofluorescence Immunofluorescent staining of microglia in the hippocampus (CA3 region) in rat brain. Floating sections are 4% paraformaldehyde perfused and stained with Rabbit Anti-Ferritin (Cat. No. F5012) and Sheep Anti-Rabbit IgG FITC (Cat. No. F7512). From Dr. S. Shoham, Herzog Hospital, Dept. of Research, Jerusalem, Israel.
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| form | liquid |
| Quality Level | 200  |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | Ouchterlony double diffusion: 1:16 |
| UniProt accession no. | P02794  |
| Application: | • Anti-Ferritin, Human antibody produced in rabbit has been used in: • immunohistochemistry • western blotting • immunocytochemical techniques |
| Application: | Anti-Ferritin, Human antibody is suitable for use in Ouchterlony double diffusion (1:16 using human spleen ferritin), and immunoelectrophoresis. The antibody may also be used in western blot (1:1000 using frozen liver homogenates) and immunoprecipitation (using 300 μg of cell lysate incubated with 6 mul of anti-ferritin for 1h at 4 ° C). |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Ferritin is an iron-storage protein that regulates iron-homeostasis in cells. While mitochondrial ferritin is a homopolymer, the cytosolic form consists of heteropolymeric L- and H-chains. Genetic mutations in ferritin L-chains have been associated with neurodegenerative disorders, whereas altered levels of ferritin have been linked to hemophagocytic lymphohistiocytosis and adult-onset Still′s disease . In immunoelectrophoresis, anti-ferritin, human antibody is specific for ferritin when used against human spleen extract and purified ferritin. No reactivity is observed against normal human serum. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ferritin, an iron-storage protein is usually present in the liver and spleen in mammals. Iron and ferritin are distributed relatively same in the eye. Ferritin is made up of heavy and light chains. |
| Immunogen: | Ferritin isolated from normal human spleen |
| Preparation Note: | The antiserum has been treated to remove lipoproteins. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |