Anti-Human IgG (Fc specific)−FITC antibody, Mouse monoclonal
SIGMA/F5016 - clone HP-6017, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Human IgG (Fc specific)
Product Type: Chemical
Particle Immunofluorescence Agarose beads coated with Human IgG probed and developed using 1:16 Monoclonal Anti-Human IgG (Fc specific)-FITC antibody produced in Mouse, Clone: Clone HP-6017 (Cat. No. F5016).
| antibody form | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | HP-6017, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, sheep, horse (IgG), goat, human |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot immunobinding: 1:16 |
| particle immunofluorescence: 1:16 |
| Application: | Monoclonal Anti-Human IgG (Fc specific)-FITC antibody produced in mouse has been used in: • Fluorescent Dot Immunobinding Assay (F-DIBA) • Particle Immunofluorescent Assay (F-IFMA) • flow cytometry |
| Biochem/physiol Actions: | Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), containing the H chain constant region. IgG antibody have enormous therapeutic potential and the Fc is for the development of therapeutic antibody. Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding. |
| Biochem/physiol Actions: | The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. The light comprises of one variable N-terminal region and a constant C-terminal region. The heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. |
| Physical form: | Solution in 0.01 M phosphate buffer pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |