Nutrient Mixture F-12 Ham
SIGMA/F6636 - powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture
Synonym: Ham’s F-12
MDL Number: MFCD00217417
Product Type: Chemical
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| components | phenol red: 0.00125 g/L L-glutamine: 0.292 g/L sodium pyruvate: 0.22 g/L glucose: 1.802 g/L |
| form | powder |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | hybridoma: suitable |
| cell culture | mammalian: suitable |
| Application: | Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse |
| Preparation Note: | Formulated to contain 11.5 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 2.68 g/L sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |