Anti-Filamin antibody,Mouse monoclonal
SIGMA/F6682 - clone PM6/317, purified from hybridoma cell culture
Synonym: Anti-ABP-280; Anti-ABPX; Anti-CSBS; Anti-CVD1; Anti-FGS2; Anti-FLN; Anti-FLN-A; Anti-FLN1; Anti-FMD; Anti-MNS; Anti-NHBP; Anti-OPD; Anti-OPD1; Anti-OPD2; Anti-XLVD; Anti-XMVD
MDL Number: MFCD00162292
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Mouse Anti-Filamin Clone: PM6/317 (Cat. No. F6682). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. HEK-293T; 3. Jurkat; 4. COS7; 5. CHO; 6. MDBK; 7. MDCK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PM6/317, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~190 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, monkey, hamster, bovine, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot (chemiluminescent): 0.5-1.0 μg/mL using whole extract of human Jurkat or HEK-293Tcells | |
| UniProt accession no. | P21333  |
| Application: | Monoclonal Anti-Filamin antibody is suitable for indirect immunofluorescence and western blot (chemiluminescent) at a concentration of 0.5-1.0μg/mL using whole extract of human Jurkat or HEK-293Tcells. |
| Application: | Monoclonal Anti-Filamin antibody produced in mouse has been used in: • immunoblotting • immunofluorescent staining • immunoprecipitation |
| Biochem/physiol Actions: | Filamin has been primarily identified from smooth muscle but it has also found in macrophages, neutrophils, platelets and other cell types. It is associated in regulation of the cytoskeletal network by cross-linking actin and linking cell membrane to the cytoskeleton. It is also linked in regulating intracellular signaling pathways, responsible for skeletal development. It binds to the ring zinc finger domain of tumor necrosis factor (TNF) and receptor-associated factor 2 (TRAF2). Filamin cross-links with actin and promotes its aggregation and gelation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Filamin, also known as actin-binding protein, is a large flexible V-shaped actin cross-linking cytoplasmic protein present in vertebrates of both muscle and non-muscle cells. It has actin-binding domains at the ends of each arm. It is a polypeptide with molecular weight of approximately 250 kDa and forms dimers in solution. It has a large flexible actin cross-linking protein present in vertebrates in both the muscle and non-muscle cells. The name filamins refers to its filamentous colocalization with actin stress fibers. It has an actin binding domain and a homodimerization domain. Native form comprises of two subunits with one actin-binding site. |
| General description: | Monoclonal Anti-Filamin (mouse IgG1 isotype) is derived from the hybridoma PM6/317 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes. |
| Immunogen: | human platelet membranes |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |