Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit
SIGMA/F6796 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-FGFR-2
MDL Number: MFCD02096345
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in Rabbit (Cat. No. F6796) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Fibroblast Growth Factor Receptor-2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with 1:10,000 Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:2,000.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human heart sections with 1:500 Rabbit Anti-Fibroblast Growth Factor Receptor-2, Extracellular (Cat. No. F6796) using Biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Formalin-fixed, paraffin-embedded section of umbilical cord stained with 1:1,000 Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in Rabbit (Cat. No. F6796) followed by Biotin (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500 Rabbit Anti-Fibroblast Growth Factor Receptor-2, Extracellular (Cat. No. F6796). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of HEK-293T cells expressing FGFR-2 were separated on SDS-PAGE and probed with Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in Rabbit (Cat. No. F6796). The antibody was developed using 1:10,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. 1:2,000 antibody untransfected 293T cells 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 115-125 kDa (doublet) |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using trypsin-digested, human and animal tissue sections |
| western blot: 1:2,000 using extract of FGFR-2 transiently transfected cells | |
| UniProt accession no. | P21802  |
| Application: | Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit has been used in immunoprecipitation and immunoblotting. |
| Biochem/physiol Actions: | Fibroblast growth factors (FGFs) are potent physiological regulators normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. FGFRs exhibit overlapping recognition and redundant specificity. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ. FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells. |
| Biochem/physiol Actions: | The antibody detects human FGFR-2 (bek) in extracts of transfected cells. |
| Biochem/physiol Actions: | The antibody specifically reacts with human FGFR-2; no reaction with FGFR-1 or FGFR-3 is detected. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Fibroblast growth factors (FGFs) belongs to the large tyrosine kinase family of growth factor receptors (17-38kDa). The FGF family consists of at least seventeen members designated FGF-1 through FGF-17 that bind to high affinity FGF receptors (FGFRs). Soluble, secreted or possibly cleaved forms of FGFR-1 and FGFR-2 have also been found in body fluids. They are glycosylated 110-150 kDa proteins that are constructed of an extracellular ligand binding region with either two, or typically three, immunoglobulin (Ig)-like domains and an eight amino acid "acidic box", a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3. |
| Immunogen: | synthetic peptide corresponding to amino acids 362-374 of the extracellular region of human FGFR-2 (Bek). |
| Packaging: | 0.2 mL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| Preparation Note: | Affinity isolated on a peptide-agarose column. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |