Monoclonal Anti-FKHR (FOXO1a) antibody produced in mouse
SIGMA/F6928 - clone FKH 117, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD03791549
Product Type: Chemical
Western Blotting Whole extract of COS-7 cells transfected with Human FKHR were separated on SDS-PAGE and probed with Monoclonal Anti-FKHR (FOXO1a) antibody produced in Mouse, Clone: FKH 117 (Cat. No. F6928). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293). Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.25 μg/mL antibody 4. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FKH 117, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 70-75 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using total extract of COS-7 cells transfected with FKHR expression plasmid | |
| UniProt accession no. | Q12778  |
| Application: | Monoclonal Anti-FKHR (FOXO1a) antibody is suitable for immunoblot assay to study the down-regulation of both p16INK4A and p14ARF expression, and immunoblot of SKMCs and HSMM cells in a study of stable ectopic expression of PAX3-FKHR in low passage primary human skeletal muscle cells. It is also suitable for microarray and indirect ELISA. |
| Application: | Monoclonal Anti-FKHR antibody has been used in: • immunoblotting • enzyme linked immunosorbent assay (ELISA) • immunoprecipitation |
| Biochem/physiol Actions: | FKHR plays a key regulatory roles in embryonic development, differentiation, apoptosis, and tumorigenesis. It was first identified at chromosomal breakpoints in human tumors and consequently linked to tumorigenesis. The activity of FKHR is regulated by growth factors via the PKB/PI3K pathway. The expression of FKHR is inhibited through phosphorylation by PKB. Within the nucleus, the dephosphorylated FKHRL1 induces expression of target genes such as Fas ligand, and triggers apoptosis. It is involved in apoptosis, glucose homeostasis, cell cycle regulation and as a nuclear receptor co factor. It has been found that FKHR also play a role in endometrial differentiation via the protein kinase A (PKA) pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | FKHR belongs to the superfamily of forkhead transcription factors (FOX). It is characterized by a common Forkhead (or winged helix) domain, a variant of the helix-turn-helix motif. FKHR contains three residues, Thr24, Ser256, and Ser319 that lie within consensus sequences for phosphorylation by protein kinase B (PKB). |
| General description: | Monoclonal Anti-FKHR (FOXO1a) (mouse IgG1 isotype) is derived from the FKH117 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human FKHR, conjugated to KLH. |
| Immunogen: | synthetic peptide corresponding to human FKHR (amino acids 636-651), conjugated to KLH. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |