Anti-Vinculin−FITC antibody, Mouse monoclonal
SIGMA/F7053 - clone hVIN-1, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Vinculin antibody produced in mouse
MDL Number: MFCD00162905
Product Type: Chemical
Immunofluorescence MDBK bovine kidney cells were fixed and permeabilized with Methanol followed by Acetone. Fixed cells were stained with 1:50 Anti-Vinculin-FITC antibody, Mouse monoclonal (Cat. No. F7053).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | hVIN-1, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 116 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | frog, chicken, mouse, canine, human, bovine, rat, turkey |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells |
| UniProt accession no. | P18206  |
| Application: | Monoclonal Anti-Vinculin-FITC antibody is suitable for immunofluorescence staining of fibroblasts cells to study the effects of nanofiber spatial organization on cell behaviors. It is also suitable for direct immunofluorescence at a dilution of 1:50 using methanol/acetone fixed cultured bovine MDBK cells. |
| Biochem/physiol Actions: | Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin. |
| Biochem/physiol Actions: | Vinculin is a cytoskeletal protein with molecular mass of ~116 kDa. Vinculin is closely associated with adhesive membrane areas, such as focal contacts, podosomes, or intercellular junctions of the zonula adherens type. It acts as a mediator in interactions of integrins and the actin cytoskeleton. It forms a bridge between integrins, receptors for ECM molecules on the cell surface and the F-actin cytoskeleton. It is associated with talin and α-actinin which directly connects with integrins. It has ability to bind α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | purified human vinculin from uterus. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |