Fluorescein isothiocyanate isomer I

SIGMA/F7250 - suitable for protein labeling, ≥90% (HPLC), powder

Synonym: FITC; Fluorescein 5-isothiocyanate

CAS Number: 3326-32-7
Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
Molecular Weight: 389.38
EC Number: 222-042-0
MDL Number: MFCD00005063
Linear Formula: C₂₁H₁₁NO₅S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-F7250-50MG	50 mg	$40.10 1/EA	Add To Favorites
45-F7250-100MG	100 mg	$60.10 1/EA	Add To Favorites
45-F7250-250MG	250 mg	$98.30 1/EA	Add To Favorites
45-F7250-500MG	500 mg	$177.00 1/EA	Add To Favorites
45-F7250-1G	1 g	$298.00 1/EA	Add To Favorites
45-F7250-5G	5 g	$1230.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F7250-100MG

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing hematology histology
assay	≥90% (HPLC)
color	orange to dark orange
fluorescence	λ_ex 492 nm; λ_em 518 nm (green)
form	powder
InChI	1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key	MHMNJMPURVTYEJ-UHFFFAOYSA-N
mp	>360 °C (lit.)
Quality Level	200
SMILES string	Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
solubility	acetone: 1 mg/mL
storage temp.	2-8°C
suitability	suitable for protein labeling
technique(s)	titration: suitable

Application:	Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)
Application:	Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC) Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.
General description:	Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm. It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.
Packaging:	1, 5 g in glass bottle
Packaging:	50, 100, 250, 500 mg in glass bottle

Symbol	GHS08
Signal word	Danger
Hazard statements	H317 - H334
Precautionary statements	P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
Hazard Codes	Xn
Risk Statements	42/43
Safety Statements	22-36/37-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90% (HPLC)
mp	>360 °C (lit.)
Storage Temp.	2-8°C
UNSPSC	12171500

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