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Fast Black K Salt hemi(zinc chloride) salt

SIGMA/F7253 - practical grade

Synonym: 2,5-Dimethoxy-4-([4-nitrophenyl]azo)benzenediazonium chloride hemi(zinc chloride) salt; Brentamine fast black K salt

CAS Number: 64071-86-9
Empirical Formula (Hill Notation): C14H12N5O4 · 0.5Cl4Zn
Molecular Weight: 417.88
EC Number: 264-656-1
MDL Number: MFCD00078471
Linear Formula: C14H12N5O4 · 1/2ZnCl4
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-F7253-25G 25 g
 $49.20
1/EA		 Add To Favorites
45-F7253-100G 100 g
 $153.00
1/EA		 Add To Favorites

 

application(s) diagnostic assay manufacturing
hematology
histology
color brown-red
composition Dye content, ≥25%
form powder
grade practical grade
InChI 1S/2C14H12N5O4.4ClH.Zn/c2*1-22-13-8-12(14(23-2)7-11(13)16-15)18-17-9-3-5-10(6-4-9)19(20)21;;;;;/h2*3-8H,1-2H3;4*1H;/q2*+1;;;;;+2/p-4
InChI key NZWZSUSPTZUGNN-UHFFFAOYSA-J
mp 150 °C
Quality Level 200 
SMILES string [Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1N=Nc2ccc(cc2)[N+]([O-])=O.COc3cc([N+]#N)c(OC)cc3N=Nc4ccc(cc4)[N+]([O-])=O
solubility water: 10 mg/mL
storage temp. room temp
technique(s) microbe id | staining: suitable
Application: Fast Black K Salt hemi (zinc chloride) salt has been used for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE. It has been used for staining endopeptidase and leucine aminopeptidase activity.

Fast black K salt has various applications as listed below-

• to stain enterochromaffin cells > as an indirect stain for periodate-generated tissue aldehyde groups
•  as a visualization agent for amphetamines and cyanobacterial toxins following their separation by thin-layer chromatography
• for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE
• staining endopeptidase and leucine aminopeptidase activity
General description: Fast black K (C.l. 37190) is a lipophilic amino-substituted monoazo dye. It is also known as Brentamine fast black K salt.
Symbol GHS07  GHS07
Signal word Warning
Hazard statements H319
Precautionary statements P305 + P351 + P338
Hazard Codes Xi
Risk Statements 36
Safety Statements 26-36
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
mp 150 °C
Storage Temp. room temp
Colour Index Number 37190
UNSPSC 12171500

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