Fructosyl-Amino Acid Oxidase from Corynebacterium sp.
SIGMA/F7296 - recombinant, expressed in E. coli, lyophilized powder, ≥0.45 units/mg protein
EC Number: 232-914-2
MDL Number: MFCD03456889
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F7296-100UN
| form | lyophilized powder |
| mol wt | ~88 kDa by electrophoresis |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| specific activity | ≥0.45 units/mg protein |
| storage temp. | −20°C |
| Application: | Fructosyl-amino acid oxidase can be used to detect the levels of glycated proteins, which are markers for diabetes mellitus. |
| Application: | Fructosyl-Amino Acid Oxidase from Corynebacterium sp has been used in glycated haemoglobin HbA1c detection in blood samples using quartz crystal microbalance (QCM) based detection. |
| Biochem/physiol Actions: | Fructosamines are formed when glucose is condensed amino group of amino acids or proteins. Fructosamine oxidases (FAOX) catalyze the oxidative deglycation of low molecular weight fructosamines. Fructosyl amino acid oxidase catalyzes the oxidation of the C-N bond linking the C1 of the fructosyl moiety and the nitrogen of the amino group of fructosyl amino acids. |
| Biochem/physiol Actions: | Fructosyl-Amino Acid Oxidase (FAOD) comprises of FAD-binding motifs and is classified into three types based on substrate specificity. The engineered Corynebacterium Fructosyl-Amino Acid Oxidase is stable at 45°C and could be exploited for the development of glycated protein biosensing system and glycated hemoglobin HbA1c measurements. FAOD shares sequence homology with fructosyl peptide oxidase and both are effective on α-fructosyl substrates. |
| Biochem/physiol Actions: | Suitable for the determination of fructosyl- |
| General description: | Enzyme Commission (E.C.) 1.5.3.x |
| General description: | Fructosyl amino acid oxidase [fructosyl-a-l-amino acid:oxygen oxidoreductase] is a flavoprotein that catalyzes the oxidation of fructosyl amino acids to form glucosone, amino acid and hydrogen peroxide. |
| Other Notes: | One unit will produce 1.0 μmole of hydrogen peroxide per minute at pH 8.0 at 37 °C. |
| Packaging: | 25, 100 units in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.45 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.5.3.x |
| UNSPSC | 12352204 |