Fluorescein diacetate
SIGMA/F7378 - used as cell viability stain
Synonym: 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein
CAS Number: 596-09-8
Empirical Formula (Hill Notation): C24H16O7
Molecular Weight: 416.38
EC Number: 209-877-6
MDL Number: MFCD00005062
Linear Formula: C24H16O7
Product Type: Chemical
| ε (extinction coefficient) | ≥86000 at 488-494 nm in 0.1 M NaOH |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | white to faint yellow |
| form | powder |
| InChI | 1S/C24H16O7/c1-13(25)28-1 |
| InChI key | CHADEQDQBURGHL-UHFFFAOYSA |
| mp | 200-203 °C (lit.) |
| Quality Level | 200 ![]() |
| SMILES string | CC(=O)Oc1ccc2c(Oc3cc(OC(C |
| solubility | acetone: 25 mg/mL |
| storage temp. | room temp |
| technique(s) | titration: suitable |
| Application: | Fluorescein diacetate has been used for the differentiation of live cells from dead cells. |
| General description: | Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. |
| General description: | Lipase substrate |
| Packaging: | 5, 10, 25, 100 g in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 200-203 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |

