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Fluorescein diacetate

SIGMA/F7378 - used as cell viability stain

Synonym: 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein

CAS Number: 596-09-8
Empirical Formula (Hill Notation): C24H16O7
Molecular Weight: 416.38
EC Number: 209-877-6
MDL Number: MFCD00005062
Linear Formula: C24H16O7
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-F7378-5G 5 g
$94.40
1/EA
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45-F7378-10G 10 g
$177.00
1/EA
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45-F7378-25G 25 g
$363.00
1/EA
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45-F7378-100G 100 g
$1040.00
1/EA
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ATR-IR Spectra for 3,6-Diacetoxyfluoran.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F7378-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F7378-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F7378-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F7378-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F7378-5G

 

ε (extinction coefficient) ≥86000 at 488-494 nm in 0.1 M NaOH
application(s) diagnostic assay manufacturing
hematology
histology
color white to faint yellow
form powder
InChI 1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
InChI key CHADEQDQBURGHL-UHFFFAOYSA-N
mp 200-203 °C (lit.)
Quality Level 200 
SMILES string CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1
solubility acetone: 25 mg/mL
storage temp. room temp
technique(s) titration: suitable
Application: Fluorescein diacetate has been used for the differentiation of live cells from dead cells.
General description: Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.
General description: Lipase substrate
Packaging: 5, 10, 25, 100 g in glass bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
mp 200-203 °C (lit.)
Storage Temp. room temp
UNSPSC 12171500

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