Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat
SIGMA/F8646 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162628
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:320 Anti-Mouse IgG (Fc specific) F(ab′)2 fragment-FITC antibody produced in Goat (Cat. No. F8646).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 |
| Application: | Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody may be used for immunohistochemistry at a working dilution of 1:320 using formalin-fixed, paraffin-embedded sections of human tonsil. Antibody dilution of 1:200 was used to label sections of human brainstems and cervical spinal cords with primary antibody against human Bcl-2 protein. The antibody was also used to label erythrocytes for photon reassignment microscopy and for FACS using melanoma cells. |
| Biochem/physiol Actions: | Binds mouse IgG; does not bind other mouse Igs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody is specific for the Fc fragment of mouse IgG. No cross reactivity is observed with the Fab fragment of mouse IgG. Purified antibody is conjugated to fluorescein isothiocyanate (FITC) in an alkaline reaction and then purified to remove unbound FITC. |
| Immunogen: | Purified mouse IgG |
| Other Notes: | Antibody adsorbed with bovine, equine, and human serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background with bovine, equine, or human samples. Useful when trying to avoid background staining due to the presence of Fc receptors. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 22-36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |