Fas/Fc Chimera from mouse
SIGMA/F8799 - >97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
Synonym: Apo-1; CD95
MDL Number: MFCD01867037
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| assay | >97% (SDS-PAGE) |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 55 kDa (glycosylated form) |
| potency | 0.03-0.1 μg per mL ED50 (in the presence of 20 ng/mL recombinant human Fas ligand for cytotoxicty assay of Jurkat cells) |
| Quality Level | 200  |
| recombinant | expressed in baculovirus infected Sf21 cells |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P25446  |
| Analysis Note: | Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells. |
| Application: | Fas/Fc Chimera from mouse has been used as a blocking agent during FACS (flow cytometry). |
| Biochem/physiol Actions: | Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS). |
| General description: | Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg bovine serum albumin per 1 μg of cytokine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |