Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit
SIGMA/F8888 - affinity isolated antibody, buffered aqueous solution
Synonym: Rabbit Anti-Chicken IgY (IgG) (whole molecule)
MDL Number: MFCD00162224
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human tonsil stained with chicken anti-human IgG followed by 1:320 Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody produced in rabbit (Cat. No. F8888).
Immunofluorescence Human periferal blood lymphoctes were stained with chicken anti-human IgG followed by 1:320 Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody produced in rabbit (Cat. No. F8888).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:320 using human peripheral blood lymphocytes |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 using human tonsil |
| Application: | Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody has been used in immunofluorescence. |
| Application: | Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody may be used for immunofluorescence using human peripheral blood lymphocytes at a working antibody dilution of 1:320. For immunohistochemistry a working antibody dilution of 1:320 may be used. A dilution of 1:1000 was used for EP receptor staining of murine keratinocyte cell line 308 using primary antibodies generated in chicken. The antibody was also used for immune electron microscopy labeling of porcine nerve samples. |
| Biochem/physiol Actions: | Binds chicken IgY (IgG). Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for chicken IgY (IgG). |
| Biochem/physiol Actions: | Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications . |
| Biochem/physiol Actions: | IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration. |
| General description: | Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa. |
| Immunogen: | chicken IgY (IgG) |
| Immunogen: | Purified chicken IgY (IgG) |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |