Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat
SIGMA/F9006 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (whole molecule)
MDL Number: MFCD00162642
Product Type: Chemical
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| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:64 |
| Application: | Anti-Mouse IgG (whole molecule)−FITC antibody produced in goat has been used in flow cytometry. |
| Application: | The antibody may be used for immunofluorescent staining of mouse spleen cells at a working dilution of 1:64. A working antibody dilution of 1:40 was used to stain the conidia of Botrytis cinerea for immunofluorescence. The antibody was also used for immunofluorescence of primary luteal cell cultures incubated with primary antibody against CD45 and rhizoids of Chara globularis incubated with anti-human spectrin antibody. |
| Biochem/physiol Actions: | IgG (immunoglobulin G) antibody has its function similar to IgM antibody in complement system activation. IgG participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody consists of four subclasses : IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa respectively. The chains are interlinked with a disulfide bond. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections Anti-Mouse IgG (whole molecule)-FITC antibody is specific for mouse IgG. Goat anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. Following conjugation, unbound FITC is removed by extensive dialysis. |
| Immunogen: | Purified mouse IgG |
| Packaging: | 0.5 mL in glass insert |
| Packaging: | 1, 2 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |