Fluorescamine
SIGMA/F9015 - ≥98% (TLC), powder, used for detection of primary amines
Synonym: 4-
CAS Number: 38183-12-9
Empirical Formula (Hill Notation): C17H10O4
Molecular Weight: 278.26
EC Number: 253-814-5
MDL Number: MFCD00005928
Linear Formula: C17H10O4
Product Type: Chemical
FT-IR Spectra for 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F9015-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F9015-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F9015-250MG
| assay | ≥98% (TLC) |
| color | off-white to yellow |
| form | powder |
| InChI | 1S/C17H10O4/c18-15-13(11- |
| InChI key | ZFKJVJIDPQDDFY-UHFFFAOYSA |
| mp | 153-157 °C (lit.) |
| SMILES string | O=C1OC2(OC=C(C2=O)c3ccccc |
| solubility | acetone: 50 mg/mL |
| storage temp. | room temp |
| Application: | Fluorescamine has been used as a stain to localize granular proteins on the surface of foxtail millet starch as well as protein in foxtail millet flour through confocal scanning laser microscopy (CSLM). It has also been used for photometric determination of para-aminobenzoic acid. |
| Application: | Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses. |
| General description: | Fluorescamine, a heterocyclic dione, reacts with primary amines, amino acids, peptides, and protein to form a fluorescent product. Excess reagent and its degradation products are non-fluorescent. The fluorescence of a solution containing protein plus fluorescamine is equivalent to the quantity of free amine groups present. This is the basic principle of a fluorescent protein assay. Fluorescamine is used as a derivatizing agent for the identification of various compounds used in the pharmaceutical industry, such as oseltamavir, penicillamine, sulfonamide residues, lisinopril, methotrexate, vigabatrin, and gabapentin. |
| Packaging: | 1 g in glass bottle |
| Packaging: | 100, 250 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (TLC) |
| mp | 153-157 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12352204 |