Anti-FRS2 (SNT-1) antibody produced in rabbit
SIGMA/F9052 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-FRS1A; Anti-FRS2A; Anti-FRS2alpha; Anti-SNT; Anti-SNT-1; Anti-SNT1
MDL Number: MFCD03791556
Product Type: Chemical
Immunoblotting PC-12 cells extract was separated on SDS-PAGE, blotted with 1:2,000 Anti-FRS2 (SNT-1) (Cat. No. F9052) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 92-95 kDa (doublet) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 10-20 μg using RIPA lysate of mouse NIH/3T3 cells |
| microarray: suitable | |
| western blot: 1:1,000 using whole cell extracts of rat PC-12 cells or human HepG2 cells | |
| UniProt accession no. | Q8WU20  |
| Biochem/physiol Actions: | Fibroblast growth factors (FGF) receptor substrate (FRS) functions as a lipid-anchored docking protein that is tyrosine phosphorylated and recruited to FGFR upon FGF stimulation. fibroblast growth factor receptor substrate 2 (FRS2) plays an important role in linking fibroblast growth factor (FGF) and nerve growth factor (NGF) with the Ras/ mitogen-activated protein kinases (MAPKs) signaling pathway, thus relaying information from the cell surface to the nucleus. FRS2 proteins are tyrosine phosphorylated in response to activation of the RET receptor, a tyrosine kinase that functions as the signal transducing receptor for the glial cell line-derived neurotrophic factor (GDNF). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Fibroblast growth factor receptor substrate 2 (FRS2), also known as suc-1 associated neurotrophic factor target protein (SNT) exist in two structurally similar forms namely, FRS2a (SNT-1) and FRS-2b (SNT-2). FRS2 is characterized with a consensus myristylation sequence, involved in its recruitment to the cell membrane 4 and a putative phosphotyrosine binding (PTB) domain in its amino-terminus. |
| Immunogen: | synthetic peptide corresponding to human FRS2 sequence, (amino acids 487-505). The corresponding sequence is identical in Xenopus and differs from the respective human and mouse FRS2β sequences by 2 and 3 amino acids, respectively. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |