Factor X Activated (Xa) from bovine plasma
SIGMA/F9302 - aqueous glycerol solution
Synonym: Factor Xa
CAS Number: 9002-05-5
MDL Number: MFCD00163610
Product Type: Chemical
Activation of factor X involves the hydrolysis of a 9.5 kDa peptide from the amino terminus of the heavy chain of the zymogen. The resulting activated factor Xaα has a MW of 45.3 kDa. Further hydrolysis of a small glycopeptide from the carboxy-terminus of the heavy chain yields factor Xaβ, which has equal coagulant activity. One disulfide bond also links the heavy and light chains of factor Xa.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F9302-50UG
| form | aqueous glycerol solution |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥50 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P00743  |
| Application: | Bovine Factor Xa is used for site specific cleavage of recombinant fusion proteins containing an accessible Factor Xa recognition site for removal of affinity tags. |
| Application: | Fusion proteins are commonly expressed with a factor Xa cleavable Ile-Glu (or Asp)-Gly-Arg-↓-X sequence. Typically 1 mg of fusion protein can be incubated with 10 μg of factor Xa for 2.5 hours at 37 °C. |
| Biochem/physiol Actions: | Factor Xa catalyzes the hydrolysis of the Arg-Thr and then Arg-Ile bonds in prothrombin to yield active thrombin. The fairly strict recognition sequence is Ile-Glu (or Asp)-Gly-Arg-↓-X. It may sometimes cleave at other basic residues, depending on the conformation of the target protein. Factor Xa will not cleave if a proline residue follows the arginine of the recognition sequence. pH Optimum: 7.6-8.0 Temperature Optimum: 37 °C |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| General description: | Factor Xa is a serine endoproteinase and a member of the S1 peptidase family. Factor Xa plays a critical role in the coagulation cascade by catalyzing the proteolytic conversion of prothrombin to active thrombin. Factor Xa′s prothrombin conversion activity is greatly enhanced in vivo when complexed with factor V, calcium ions and phospholipids on the activated platelet surface. The zymogen form, Factor X, is activated in vivo by two different pathways. The intrinsic pathway utilizes a catalytic complex composed of factor IXa, factor VIII, phospholipids and calcium ions. The extrinsic pathway utilizes a complex of factor VII and tissue factor. The factor X zymogen is a 55 KDa glycoprotein with a light and heavy chain joined by a single disulfide. |
| Other Notes: | One unit of activated Factor X will liberate 1.0 μmole of p-nitroanilide from N-benzoyl- |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.6 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |