Anti-Human IgG (Fc specific)−FITC antibody produced in goat
SIGMA/F9512 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti Human IgG Fitc - Anti-Human IgG (Fc specific)-FITC antibody produced in goat; Anti Human Igg Fitc
MDL Number: MFCD00162438
Product Type: Chemical
Immunofluorescence Hep2 human carcinoma cells were stained with human anti-nuclear (A.N.A.) followed by 1:128 Anti-Human IgG (Fc specific)-FITC antibody produced in Goat (Cat. No. F9512).
Immunofluorescence Human periferal blood lymphoctes were stained with1:16 Anti-Human IgG (Fc specific)-FITC antibody produced in Goat (Cat. No. F9512) .
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 1:64-1:128 using Hep2 cells |
| Application: | Anti-Human IgG (Fc specific)-FITC antibody produced in goat has been used in: • immunofluorescence • immunohistochemistry • surface labeling experiments |
| Biochem/physiol Actions: | Binds human IgG; does not bind other human Igs. Provides reduced background with mouse or rat samples |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections Anti-Human IgG (Fc specific)-FITC antibody specifically recognizes Fc fragment of all human IgG subclasses. The conjugate shows no reactivity with human IgG Fab fragment, IgA, IgM and light chains and normal mouse or rat serum proteins. The antibody is purified by immunospecific purification and then conjugated to FITC. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. The antibody is isolated from goat anti-human IgG antiserum by immunospecific purification, which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG. |
| Immunogen: | Purified human IgG Fc fragment |
| Other Notes: | No cross-reaction with mouse and rat IgG. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |