Anti-Rabbit IgG (whole molecule)–FITC antibody produced in goat
SIGMA/F9887 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Rabbit IgG (whole molecule)
MDL Number: MFCD00162786
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human tonsil stained with Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635) followed by 1:160 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Immunofluorescence HeLa cells treated with 1 μg/mL staurosporine were fixed and permeabilized with Methanol followed by Acetone. Fixed cells were stained with 1:1,000 Anti-Caspase 3, Active antibody produced in Rabbit (Cat. No. C8487). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887). Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | FITC conjugate |
| contains | ≥15 mM sodium azide as preservative |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:160 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 |
| Application: | Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat has been used in immunohistochemistry and indirect immunofluorescence. |
| Biochem/physiol Actions: | Binds all rabbit Igs. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections IgG3 mediates effector functions IgG4 are associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region is involved for the development of therapeutic antibody. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. |
| General description: | There are four classes of IgG namely IgG1, IgG2, IgG3, and IgG4. Among them IgG1 is the most abundant antibody. |
| Immunogen: | Purified rabbit IgG |
| Other Notes: | Antibody adsorbed with human IgG. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, with 15 mM sodium azide as a preservative. |
| Preparation Note: | Adsorbed to reduce background staining with human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |