Fructose Assay Kit
SIGMA/FA20 - sufficient for 20 assays
Synonym: Fructose Test Kit
EC Number: 200-333-3
Product Type: Chemical
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| analyte chemical class(es) | sugars (fructose) |
| application(s) | food and beverages general analytical |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | photometry: suitable |
| usage | sufficient for 20 assays |
| Application: | This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12164500 |
| Components | fructose standard 500 mg; glucose assay reagent 1 mL/vial; phosphoglucose isomerase 1 mL/vial |