Fluorescein isothiocyanate–dextran

SIGMA/FD250S - average mol wt 250,000

Synonym: FITC–Dextran

CAS Number: 60842-46-8
MDL Number: MFCD00131092
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-FD250S-100MG	100 mg	$145.00 1/EA	Add To Favorites
45-FD250S-250MG	250 mg	$341.00 1/EA	Add To Favorites
45-FD250S-1G	1 g	$835.00 1/EA	Add To Favorites

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This image depicts SKU: FD250S-100MG

Properties
Descriptions
Safety Info.
Basic Data

biological source	bacterial (Leuconostoc mesenteroides)
color	yellow to orange
conjugate	FITC conjugate
extent of labeling	0.003-0.020 mol FITC per mol glucose
form	powder
mol wt	average mol wt 250,000
Quality Level	200
solubility	water: 25 mg/mL, clear, yellow
storage temp.	2-8°C

Application:	Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
Application:	FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
Application:	FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis. FITC-Dextran 250 (FD 250) may be used as a model compound for development of methods to deliver molecules into protoplasts and to study drug release from structures such as hydrogels and liposomes. It may be used as a model compound to study microvasculature and blood brain barrier.
General description:	Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
Other Notes:	To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
Packaging:	1 g in poly bottle
Packaging:	100, 250 mg in poly bottle
Preparation Note:	Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Symbol	GHS07
Signal word	Warning
Hazard statements	H315 - H319 - H335
Precautionary statements	P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352201

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