Free Glycerol Determination Kit
SIGMA/FG0100 - 1 kit sufficient for 1000 reactions
Synonym: Triglyceride and Free Glycerol Kits and Reagents
Product Type: Chemical
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| detection method | colorimetric |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| usage | kit sufficient for 1000 reactions |
| Application: | Suitable for the quantitative enzymatic determination of glycerol in serum or plasma. |
| Application: | The Free Glycerol Determination Kit has been used to determine the level of glycerol released into the medium from mammalian cells grown in vitro . |
| Application: | The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample. |
| Application: | The triglyceride and free glycerol kits are for the quantitative determination of glycerol, total triglycerides or free triglycerides. |
| General description: | Glycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification. In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed. |
| Other Notes: | In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed. |
| Symbol |    GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H410 |
| Precautionary statements | P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn,N |
| Risk Statements | 22-36-51/53 |
| Safety Statements | 26-61 |
| RIDADR | UN 3077 9 / PGIII |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |