Adenosine 5′-triphosphate (ATP) assay mix dilution buffer
SIGMA/FLAAB - lyophilized powder
Synonym: ATP Assay Dilution Buffer; Assay Mix Buffer; Assay Mix for ATP
Product Type: Chemical
ATP is hydrolyzed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. Reaction (1) is reversible and the equilibrium lies far to the right. Reaction (2) is essentially irreversible. When ATP is the limiting reagent, the light emitted is proportional to the ATP present.
| form | lyophilized powder |
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | activity assay: suitable |
| Application: | Adenosine 5′-triphosphate (ATP) assay mix dilution buffer has been used to determine the level of adenosine kinase activity in isolated sections of mouse hippocampi. It has also been used to measure the ATP content in oocytes and harderian gland(HG) tissue homogenates. |
| General description: | Adenosine 5′-triphosphate (ATP) assay mix dilution buffer is a component of the ATP bioluminescent assay kit that may be employed for the quantitative bioluminescent determination of ATP in experimental samples. |
| Preparation Note: | Adenosine 5′-triphosphate (ATP) assay mix dilution buffer contains MgSO4, dithiothreitol (DTT), ethylene diamine tetraacetic acid (EDTA, bovine serum albumin (BSA), and tricine buffer salts. |
| Preparation Note: | Reconstitute with 50 mL of sterile water. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H318 |
| Precautionary statements | P280 - P305 + P351 + P338 + P310 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |