Adenosine 5′-triphosphate (ATP) assay mix
SIGMA/FLAAM - lyophilized powder
Synonym: ATP assay kit; ATP detection kit
Product Type: Chemical
ATP is hydrolyzed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. Reaction (1) is reversible and the equilibrium lies far to the right. Reaction (2) is essentially irreversible. When ATP is the limiting reagent, the light emitted is proportional to the ATP present.
| form | lyophilized powder |
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| usage | 1 vial sufficient for ≥50 assays |
| Application: | Adenosine 5′-triphosphate (ATP) assay mix has also been used to measure the level of cellular ATP in ATP viability assay. |
| Application: | The ATP assay kit has been used to measure the level of cellular ATP present in isolated mitochondrial fractions from L. donovani promastigotes. |
| General description: | The Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of adenosine 5’-triphosphate (ATP) in samples containing 2x10–12 to 8x10–5 moles/liter. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin. |
| Other Notes: | Contains luciferase, luciferin, MgSO4, EDTA, DTT, BSA, and Tricine buffer. |
| Preparation Note: | Reconstitute with 5 ml of sterile water. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H318 |
| Precautionary statements | P280 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |