Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit
SIGMA/FLASC - for cellular ATP determination
Synonym: ATP bioluminescence assay kit
Product Type: Chemical
ATP is hydrolyzed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. Reaction (1) is reversible and the equilibrium lies far to the right. Reaction (2) is essentially irreversible. When ATP is the limiting reagent, the light emitted is proportional to the ATP present.
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | The Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit has been used to determine accurately the level of ATP present in neutrophils isolated from the blood of over 100 human test patients. |
| General description: | Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit may be employed for the bioluminescent determination of adenosine 5′-triphosphate (ATP) released from a suspension of viable somatic cells. Estimates of cell concentrations may be calculated if it is assumed that the ATP content per viable cell remains constant. The number of viable somatic cells are selectively counted because, as a cell dies, its ATP is rapidly degraded. |
| Symbol |   GHS05,GHS09 |
| Signal word | Danger |
| Hazard statements | H318 - H410 |
| Precautionary statements | P273 - P280 - P305 + P351 + P338 - P391 - P501 |
| Hazard Codes | Xi |
| Risk Statements | 36-52/53 |
| Safety Statements | 26-61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |
| Components | FL-AAB Dilution buffer 1 mL/vial; FL-AAM Assay mix 1 mL/vial; FL-AAS ATP standard 1 mL/vial; FL-SAR Somatic cell releasing reagent 1 mL/vial |