Firefly Lantern Extract
SIGMA/FLE250 - for ATP determination
Synonym: FLE
Product Type: Chemical
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| form | solution |
| packaging | pkg of 250 mg dried lanterns |
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | toxicology assay: suitable |
| Application: | Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities. |
| Application: | Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells. |
| Biochem/physiol Actions: | Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin. |
| General description: | The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif. |
| Other Notes: | Crude luciferin and luciferase |
| Packaging: | 1 vial in serum bottle |
| Preparation Note: | After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO4. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |